Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon.

A mouse cDNA sequence for epididymal androgen-regulated proteins related to glutathione peroxidase.

A cDNA was isolated from a murine epididymal cDNA library REFERENCES in Xgtll by differential hybridization. This cDNA codes for , „, ,. , .. D ,. „ , f . . . . . _ c IT1 / i n n n . , _ .. . ? , , . . . . , , ,s m '• Ghyselinck.N.B., Jimenez.C, Lefrancois.A.M. and DufaureJ.P. (1990)/ 24kDa androgen-regulated epididymal secretory proteins (1). The M^ gru^r 4 5-12 sequence is presented below, al...

Glutathione peroxidase activity in cell cultures from different regions of human epididymis.

AIM To study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis. METHODS Tissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetr...

Analysis of eukaryotic mRNA structures directing cotranslational incorporation of selenocysteine.

Translation of an mRNA encoding a selenoprotein requires that at least one UGA codon in the reading frame is recoded as a site for the insertion of selenocysteine. In eukaryotes, the termination codon recoding event is directed by a cis-acting signal element located in the 3' untranslated region of the gene. This 'selenocysteine insertion sequence' (SECIS) comprises conserved sequences in a reg...

A selenoprotein in the plant kingdom. Mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chlamydomonas reinhardtii gluththione peroxidase.

Selenoproteins that contain the rare amino acid selenocysteine in their primary structure have been identified in diverse organisms such as viruses, bacteria, archea, and mammals, but so far not in yeast or plants. Among the most thoroughly investigated families of selenoenzymes are the animal glutathione peroxidases (GPXs). In the last few years, genes encoding GPX-like homologues from Chlamyd...

A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chlamydomonas reinhardtii glutathione peroxidase